- Once the reference and the dark data are captured. Tap the “Method Builder” tab on the right panel.
- Place the cuvette that contains the sample into the cuvette holder.
- Click the Capture button to collect the spectrum data.
- Enter the concentration value for the sample in the text field.
- Click the “Add Data” button to add the sample to the method builder.
- Repeat Steps 2 through 5 until all the desired samples are added.
- Click the “Create Method” button to create a method using the added data.
- In the popup window, enter the wavelength that needs to be monitored as well as the method name. Then click the “Save” button to compute and save the method.
- Computed slope, constant and coefficient of determination (R square) will be displayed for the goodness of the method.
- Newly created method will be automatically loaded for the prediction. If not, saved method can be loaded in the App Setup as well in by tap on the “Model” in the Predictor tab.
- Place the unknown concentration sample into the cuvette holder.
- Navigate to “Predictor” tab, and press the Capture button to make the concentration prediction.
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