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For clarity, “Dark” will be used to indicate a SpectraWiz dark spectrum and “dark spectrum” will refer to the concept. When you acquire a Dark, the SpectraWiz software saves the spectrum and subtracts it from all subsequent spectra that are acquired. Because of this mechanism, the Dark can be used for multiple purposes. For instance, a Dark can be taken while ambient light sources are on. Then the Dark is technically a background spectrum. This is illustrated below. Here is the spectrum of a white light source with ambient sources of light present:

Here is the spectrum of just the ambient lights. This is the spectrum to be used as a Dark:

This is the final spectrum after the Dark is subtracted: