Akiko Sunada, Tomosumi Kamimura, and Ryohei Yasukuni | Journal of Laser Micro Nanoengineering | August 1, 2026 | 10.2961/jlmn.2026.02.2009
Abstract
The complex synthesis of nanopharmaceuticals still faces hurdles regarding stability, sterilization, storage, and quality control toward industrial production of poorly water-soluble drug formulations. In this study, we investigated the versatility of bovine serum albumin (BSA) as a biocompatible dispersing agent for nanoparticles produced by Pulsed Laser Fragmentation in Liquid (PLFL). We selected quinacridone (QA) as a model compound of nanomedicine due to its low topological polar surface area (tPSA = 58.2 Ų), representing a higher degree of hydrophobicity that typically face solubility problem in gastrointestinal fluids. PLFL of QA in BSA solution yielded finer nanoparticles with enhanced fragmentation efficiency compared to those in water. While QA nanoparticles produced in water agglomerated immediately in phosphate-buffered saline (PBS), nanoparticles exhibited long-term dispersion stability in the presence of BSA. Furthermore, fluorescence spectroscopy revealed molecular-like emission bands only with BSA, suggesting that strong hydrophobic interactions disturbed the surface molecular arrangement. These results demonstrate that BSA can serve an effective dispersing agent for low tPSA compounds.
StellarNet equipment used: EPP2000
How it was used: used The spectrometer recorded fluorescence from the quinacridone nanoparticles and helped identify emission changes produced by BSA stabilization.
